All specimens can be stored at 2-30°C

High Risk HPV Testing by Hologic Panther

If you currently are not a reflexive HPV account, of if you are unsure, please check any red boxes on the requisition, in this section, that apply to the particular request.

If a ThinPrep® test was submitted on the patient, it can be used for this test also, provided:

1. The test is ordered within 105 days of the collection date.
2. There is sufficient volume to complete the additional test.
3. No other chemicals (such as acetic acid) have been added to the specimen.

If a conventional Pap test was submitted, or no Pap test is necessary, collect a sample as indicated for the ThinPrep® Pap test, but mark “HPV Testing” and “NO PAP” boxes on the requisition.

HPV/16/18/45 Genotyping by Hologic Panther

1. Available on request
2. Justifiable reflexive on any positive High Risk HPV test result
3. Processed from current HPV test vial.

If interested in establishing reflexive HPV or Genotype testing on your patient’s abnormal Pap results, please contact our office at 252-561-7992, extension 244.

Chlamydia and/or Gonorrhea Testing by Hologic Panther

Please check any red boxes on the requisition, in this section, that apply to the particular request.

1. The test is ordered within 30 days of the collection date.
2. There is sufficient volume to complete the additional test.
3. No other chemicals (such as acetic acid) have been added to the specimen.

*If no ThinPrep® Pap Test is required, these alternative collection techniques may be utilized:

1. Aptima Unisex Swab Collection Kit (white label).
   1. Submit blue swab only.
   2. Testing must be performed within 60 days of collection.
   3. Refer to kit packaging for collection instructions
2. A urine sample may be submitted, provided:
• 20-30 ml of first catch urine is collected in a preservative-free collection cup.
• The collection was performed within 24 hours of testing

                Or

• Aptima Urine Transport Collection Kit is utilized (yellow tube).
• Testing is performed 30 days of collection.
• Refer to kit packaging for collection instructions.

*For use with Male or Female Patients

Trichomonas Vaginalis Testing by Hologic Panther

If a ThinPrep® Pap test was submitted on the patient, it may be used for this test also, provided:

1. The test is performed within 30 days of collection.
2. There is sufficient volume to complete testing.
3. No other chemicals (such as acetic acid) have been added to the specimen.
4. TV, Trich or Trichomonas Vaginalis is written at the top of the Greenville Pathology, P.A. requisition or the appropriate box is selected, if applicable.

*If no ThinPrep® Pap Test is required, these alternative collection techniques may be utilized:

1. Aptima Unisex Swab Collection Kit (white label)
   1. Submit blue swab only.
   2. Testing must be performed within 60 days of collection.
   3. Refer to kit packaging for collection instructions.
2. A urine sample may be submitted, provided:
• 20-30 ml of first catch urine is collected in a preservative-free collection cup.
• The collection was performed within 24 hours of testing

Or

• Aptima Urine Transport Collection Kit is utilized (yellow tube).
• Testing is performed 30 days of collection.
• Refer to kit packaging for collection instructions.

*For use with Male or Female Patients

Bladder Cancer Screening

Protocol for Bladder Cancer screening by FISH studies for chromosomes 3,7, and 17 aneuploidy and 9p21 deletion:

1. Collection of 35 ml of voided urine in either:
   1. A prefilled sterile urine cup provided by Greenville Pathology, P.A. at room temperature.
   2. A preservative-free sterile urine cup provided by Greenville Pathology, P.A. stored at ˜5°C.
2. Testing should be performed within 48 hours of collection.
3. Note FISH for bladder cancer screening on the “additional tests” section of a Greenville Pathology P.A. for requisition.
4. If routine urine cytology is also requested, send the remaining urine to Greenville Pathology, P.A.for processing and note “urine cytology” on the requisition form.  There is no reason to fill out an additional Greenville Pathology, P.A. requisition.

Herpes Simplex Virus Type 1/2(HSV) Testing by PCR/TMA

Please check all appropriate red boxes on requisition or note request on order.

If a ThinPrep® Pap is requested, HSV testing may also be performed from the same vial, if:

1. Sufficient volume remains for testing.
2. No other chemicals or lysing agents have been added to the vial.
3. Testing is performed within 7 days of collection.

If no Pap is requested, the following collection devices may be utilized:

1. Aptima unisex collection kit (white label).
   1. Please refer to package label for collection instructions.
   2. Any specimen submitted with no blue swab or a cleaning swab present will be rejected.
   3. Testing must be performed within 7 days of collection.
2. Aptima Vaginal Collection Kit (orange label).
   1. Please refer to package label for collection instructions.
   2. Testing must be performed within 7 days of collection

Bacterial Vaginosis by NAA (Nucleic Acid Amplification)

Please request by requisition free text or test notation

1. Aptima Unisex Collection Kit (white label).
   1. Please refer to package label for collection instructions.
   2. Any specimen submitted with no blue swab or cleaning swab present will be rejected.
   3. Testing must be performed within 30 days of collection.
2. Aptima Vaginal Collection Kit (orange label).
   1. Please refer to package label for collection instructions.
   2. Testing must be performed within 30 days of collection.